|
KMID : 0811720190230020151
|
|
Korean Journal of Physiology & Pharmacology
2019 Volume.23 No. 2 p.151 ~ p.159
|
|
|
Prediction of itching diagnostic marker through RNA sequencing of contact hypersensitivity and skin scratching stimulation mice models
|
|
Kim Young-Won
Zhou Tong
Ko Eun-A
Kim Seong-Tae
Lee Dong-Hee
Seo Ye-Lim
Kwon Na-Hee
Choi Tae-Yeon
Lim Hee-Jung
Cho Sung-Vin
Bae Gwan-Hui
Hwang Yu-Seong
Kim Do-Jin
Park Hye-Won
Lee Min-Jae
Jang Eun-Kyung
Choi Jeong-Yoon
Bae Hye-Mi
Lim In-Ja
Bang Hyo-Weon
Ko Jae-Hong
|
|
|
Abstract
|
|
|
|
Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a, Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.
|
|
|
KEYWORD
|
|
|
Cytokines, Pruritus, RNA sequence analysis, Transient receptor potential channels, Wound healing
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|